BIN1 Protein, Human, Recombinant (His Tag)

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BIN1 Protein, Human, Recombinant (His Tag): Product Information

Purity
> 85 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human BIN1 isoform BIN1-13 (O00499-10) (Met 1-Pro 424) was expressed, with a polyhistidine tag at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human BIN1 comprises 439 amino acids and has a predicted molecular mass of 49.3 kDa. It migrates as an approximately 52 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 10% glycerol, 1mM DDT, pH 8.0
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

BIN1 Protein, Human, Recombinant (His Tag): Images

BIN1 Protein, Human, Recombinant (His Tag): Synonyms

AMPH2 Protein, Human; AMPHL Protein, Human; SH3P9 Protein, Human

BIN1 Background Information

Amphiphysin 2, also named bridging integrator-1 (BIN1) or SH3P9, Amphiphysin2/BIN1 is a crescent-shaped N-BAR protein playing a key role in forming deeply invaginated tubes in muscle T-tubules, has been recently implicated in rare and common diseases affecting different tissues and physiological functions. BIN1 downregulation is linked to cancer progression and also correlates with ventricular cardiomyopathy and arrhythmia preceding heart failure. Increased BIN1 expression is linked to increased susceptibility for late-onset Alzheimer's disease. In addition, altered splicing may account for the muscle component of myotonic dystrophies, while recessive germinal mutations cause centronuclear myopathy. Bridging integrator 1 (BIN1) as a late-onset Alzheimer's disease (AD) susceptibility gene. There are at least 15 different known isoforms of BIN1, with many being expressed in the brain including the longest isoform (iso1), which is brain-specific and localizes to axon initial segments and nodes of Ranvier. The bridging integrator 1 (BIN1) tumor suppressor encodes multiple alternatively spliced isoforms implicated in DNA repair, cell-cycle control, apoptosis and membrane dynamics.
Full Name
bridging integrator 1
Research Areas
References
  • Eisenman RN. (2001) Deconstructing Myc. Genes & Dev. 15: 2023-30
  • Bouchard C, et al. (1998) Control of cell proliferation by Myc. Trends in Cell Biology. 8(5): 202-6.
  • Sakamurol D, et al. (1996) BIN1 is a novel MYC-interacting protein with features of a tumour suppressor. Nature Genetics. 14: 69-77.
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