ACE2 Protein, Human, Recombinant (mFc Tag)

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ACE2 Protein, Human, Recombinant (mFc Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
Measured by its ability to cleave a fluorogenic peptide substrate, McaYVADAPK (Dnp)OH, R&D Systems, Catalog # ES007. The specific activity is >500 pmols/min/ug.
Protein Construction
A DNA sequence encoding the human ACE2 (NP_068576.1) (Met1-Ser740) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 18
Molecule Mass
The recombinant human ACE2 consists 957 amino acids and predicts a molecular mass of 110 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ACE2 Protein, Human, Recombinant (mFc Tag): Images

Loaded Human ACE2 Protein (Fc tag) (Cat.No.10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombiant Protein (Cat.No: 40591-V08H) with an affinity constant of 18.6 nM as determined by Octet RED System.

ACE2 Protein, Human, Recombinant (mFc Tag): Synonyms

ACEH Protein, Human

ACE2 Background Information

Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang-(1-9), which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang-(1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes, and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.
Full Name
angiotensin I converting enzyme 2
References
  • Koitka A, et al. (2008) Angiotensin converting enzyme 2 in the kidney. Clin Exp Pharmacol Physiol. 35(4): 420-5.
  • Raizada MK, et al. (2007) ACE2: a new target for cardiovascular disease therapeutics. J Cardiovasc Pharmacol. 50(2): 112-9.
  • Imai Y, et al. (2007) Angiotensin-converting enzyme 2 (ACE2) in disease pathogenesis. Circ J. 74(3): 405-10.
  • Turner AJ, et al. (2004) ACE2: from vasopeptidase to SARS virus receptor. Trends Pharmacol Sci. 25(6): 291-4.
  • Isolation of a human monoclonal antibody specific for the receptor binding domain of SARS-CoV-2 using a competitive phage biopanning strategy
    Author
    Zeng, X;Li, L;Lin, J;Li, X;Liu, B;Kong, Y;Zeng, S;Du, J;Xiao, H;Zhang, T;Zhang, S;Liu, J;
    Year
    2020
    Journal
    Antibody Therapeutics
  • SARS-CoV-2 assays to detect functional antibody responses that block ACE2 recognition in vaccinated animals and infected patients
    Author
    Walker, S;Chokkalingam, N;Reuschel, E;Purwar, M;Xu, Z;Gary, E;Kim, K;Schultheis, K;Walters, J;Ramos, S;Smith, T;Broderick, K;Tebas, P;Patel, A;Weiner, D;Kulp, D;
    Year
    2020
    Journal
    bioRxiv
  • High potency of a bivalent human VH domain in SARS-CoV-2 animal models
    Author
    Li, W;Schäfer, A;Kulkarni, S;Liu, X;Martinez, D;Chen, C;Sun, Z;Leist, S;Drelich, A;Zhang, L;Ura, M;Berezuk, A;Chittori, S;Leopold, K;Mannar, D;Srivastava, S;Zhu, X;Peterson, E;Tseng, C;Mellors, J;Falzarano, D;Subramaniam, S;Baric, R;Dimitrov, D;
    Year
    2020
    Journal
    Cell
  • Enhanced elicitation of potent neutralizing antibodies by the SARS-CoV-2 spike receptor binding domain Fc fusion protein in mice
    Author
    Liu, X;Drelich, A;Li, W;Chen, C;Sun, Z;Shi, M;Adams, C;Mellors, J;Tseng, C;Dimitrov, D;
    Year
    2020
    Journal
    Vaccine
    Application
    WB; FCM; ELISA;
  • A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2)
    Author
    Lee, J;Choi, M;Jung, Y;Lee, S;Lee, C;Kim, J;Kim, J;Kim, N;Kim, B;Kim, H;
    Year
    2020
    Journal
    Biosensors and Bioelectronics
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