Human coronavirus (HCoV-229E) Nucleoprotein / NP Protein (His Tag)

1/1
Price:
Size:
Number:

Human coronavirus (HCoV-229E) Nucleoprotein / NP Protein (His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE.
Endotoxin
Please contact us for more information.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the Human coronavirus (HCoV-229E) Nucleoprotein (NP_073556.1) (Met1-Asn389) was expressed with a polyhistide tag at the N-terminus.
Expressed Host
E. coli
Species
HCoV-229E
Predicted N Terminal
Met
Molecule Mass
The recombinant heterodimer of Human coronavirus (HCoV-229E) Nucleoprotein consists of 396 amino acids and has a calculated molecular mass of 44.43 KDa.
Formulation
Lyophilized from sterile 40mM PB, 500mM NaCl, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Human coronavirus (HCoV-229E) Nucleoprotein / NP Protein (His Tag): Images

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.