Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin / HA Protein (His Tag)


Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin / HA Protein (His Tag): Product Information

> 97 % as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
Testing in progress
Protein Construction
A DNA sequence encoding the extracellular domain of Influenza A virus H9N2 hemagglutinin (Influenza A virus (A/Hong Kong/1073/99 (H9N2)) (NP_859037.1) (Met 1-Lys 523) (HA1+HA2, uncleaved) was fused with a polyhistidine tag at the C-terminus.
Expressed Host
HEK293 Cells
Predicted N Terminal
Asp 19
Molecule Mass
The secreted recombinant hemagglutinin of Influenza A virus H9N2 (A/Hong Kong/1073/99) comprises 516 amino acids with the predicted molecular mass of 58 kDa. As a result of glycosylation, it migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin / HA Protein (His Tag): Images

Hemagglutinin/HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
  • White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
  • Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
  • Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018

H5N1 Hemagglutinin/HA Proteins

H1N1 Hemagglutinin/HA Proteins

H3N2 Hemagglutinin/HA Proteins

  • Label-free detection and characterization of the binding of hemagglutinin protein and broadly neutralizing monoclonal antibodies using terahertz spectroscopy
    Sun, Y;Zhong, J;Zhang, C;Zuo, J;Pickwell-MacPherson, E;
    J Biomed Opt
  • Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza
    Anderson, AM;Baranowska-Hustad, M;Braathen, R;Grodeland, G;Bogen, B;
    J. Immunol.
  • Induction of cross-group broadly reactive antibody response by natural H7N9 avian influenza virus infection and immunization with inactivated H7N9 vaccine in chickens
    Hu, Z;Shi, L;Xu, N;Wang, X;Hu, J;Zhao, J;Liu, X;Hu, S;Gu, M;Cao, Y;Liu, X;
    Transbound Emerg Dis
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