CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & AVI Tag)

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CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & AVI Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Labeled biotin to CD32B / Fcgr2b Protein, Cynomolgus, Recombinant (His & AVI Tag) by a certain molar ratio;
Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Cynomolgus, Recombinant (His & AVI Tag), Biotinylated (Cat. 90014-C27H-B) bound to IgG1 Antibody was 6.1 nM.
Protein Construction
A DNA sequence encoding the cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Gly 220) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Cynomolgus
Predicted N Terminal
Ala 45
Molecule Mass
The recombinant cynomolgus CD32b consists of 211 amino acids and has a calculated molecular mass of 23.5 kDa. The apparent molecular mass of the recombinant protein is approximately 33-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & AVI Tag): Images

Labeled biotin to CD32B / Fcgr2b Protein, Cynomolgus, Recombinant (His & AVI Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Cynomolgus, Recombinant (His & AVI Tag), Biotinylated (Cat. 90014-C27H-B) bound to IgG1 Antibody was 6.1 nM.

CD32B/Fcgr2b Background Information

Full Name
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
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