IL-33 Protein, Canine, Recombinant

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IL-33 Protein, Canine, Recombinant: Product Information

Purity
> 97 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Canine IL33 (Cat:70005-DNAE) at 10 μg/ml (100 μl/well) can bind human IL1R4-Fc (Cat:10105-H02H), The EC50 of human IL1R4-Fc (Cat:10105-H02H) is 0.25-0.66 μg/ml.
Protein Construction
A DNA sequence encoding the mature form of canine IL33 (O97863) (Ser 110-Ser 263) was expressed, with an initial Met at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Canine
Predicted N Terminal
Met
Molecule Mass
The recombinant canine IL33 consists of 155 amino acids and has a calculated molecular mass of 17.8 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 19KDa band.
Formulation
Lyophilized from sterile PBS, pH7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-33 Protein, Canine, Recombinant: Images

Measured by its binding ability in a functional ELISA. Immobilized Canine IL33 (Cat: 70005-DNAE) at 10 μg/ml (100 μl/well) can bind human IL1R4-Fc (Cat: 10105-H02H), The EC50 of human IL1R4-Fc (Cat: 10105-H02H) is 0.25-0.66 μg/ml.

IL-33 Background Information

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.
Full Name
interleukin 33
Related Pathways
  • IL1 signaling pathway
    IL1 signaling pathway
References
  • Iikura M, et al. (2007) IL-33 can promote survival, adhesion and cytokine production in human mast cells. Lab Invest. 87(10): 971-8.
  • Lamkanfi M, et al. (2009) IL-33 raises alarm. Immunity. 31(1): 5-7.
  • Elevated circulating Th2 but not group 2 innate lymphoid cell responses characterize canine atopic dermatitis
    Author
    Früh, S;Saikia, M;Eule, J;Mazulis, C;Miller, J;Cowulich, J;Oyesola, O;Webb, L;Peng, S;Cubitt, R;Danko, C;Miller, W;Tait Wojno, E;
    Year
    2020
    Journal
    Veterinary Immunology and Immunopathology
    Application
    cell-based
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