SARS-CoV-2 (2019-nCoV) NSP8-AVI Recombinant Protein

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SARS-CoV-2 (2019-nCoV) NSP8-AVI Recombinant Protein: Product Information

Purity
> 90 % as determined by SDS-PAGE.
Endotoxin
Please contact us for more information.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) NSP8 Protein (YP_009725304.1) (Ala1-Gln198) was expressed with a AVI tag at the C-terminus.
Expressed Host
E. coli
Species
2019-nCoV
Predicted N Terminal
Gly
Molecule Mass
The recombinant SARS-CoV-2 (2019-nCoV) NSP8 consists of 220 amino acids and predicts a molecular mass of 24.17 kDa.
Formulation
Lyophilized from sterile 20mM Tris 500mM NaCl pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SARS-CoV-2 (2019-nCoV) NSP8-AVI Recombinant Protein: Images

Coronavirus NSP8 Background Information

NSP8 is a nonstructural protein of coronavirus. NSP8 acts as a primase in RNA synthesis. NSP8 and NSP7 are essential co-factors of NSP12 (the catalytic subunit with RNA-dependent RNA polymerase activity) that can remarkably stimulates RdRp activity. The nsp12-nsp7-nsp8 subcomplex is defined as the minimal core component for mediating coronavirus RNA synthesis.
References
  • Qi Peng,et al.Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2.Cell Reports.2020
  • Yan Gao,et al.Structure of the RNA-dependent RNA polymerase from COVID-19 virus.Science.2020
  • Purnima Kumar,et al.The nonstructural protein 8 (nsp8) of the SARS coronavirus interacts with its ORF6 accessory protein.Virology.2007
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