SARS-CoV-2 (2019-nCoV) 3CLpro / 3C-like protease-His & AVI Recombinant Protein
Cat: 40594-V56B
SARS-CoV-2 (2019-nCoV) 3CLpro / 3C-like protease-His & AVI Recombinant Protein: Product Information
Purity
> 90 % as determined by SDS-PAGE.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
Measured by its ability to cleave a peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans.The specific activity is >3 pmols/min/μg
Protein Construction
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) 3CLpro (YP_009725295.1) (Ser3264-Gln3569) was expressed with a N-terminus polyhistide tag and AVI tag at the N-terminus.
Expressed Host
Baculovirus-Insect Cells
Species
2019-nCoV
Predicted N Terminal
Met
Molecule Mass
The recombinant heterodimer of SARS-CoV-2 (2019-nCoV) 3CLpro consists of 332 amino acids and has a calculated molecular mass of 37.12 KDa.
Formulation
Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH 8.0
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SARS-CoV-2 (2019-nCoV) 3CLpro / 3C-like protease-His & AVI Recombinant Protein: Images
CoV 3CLpro / 3C-like protease Background Information
3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site.
References
- Tomonari Muramatsu,et al.Autoprocessing mechanism of severe acute respiratory syndrome coronavirus 3C-like protease (SARS-CoV 3CLpro) from its polyproteins.FEBS Journal.2013
- Ziebuhr J,Molecular biology of severe acute respiratory syndrome coronavirus. Curr Opin Microbiol.2004
- Yang H, et al. The crystal structures of severe acute respiratory syndrome virus main protease and its complex with an inhibitor. Proc Natl Acad Sci USA.2003
2019-nCoV CoV 3CLpro / 3C-like protease Proteins
