Anti-RBP4 Antibody

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Anti-RBP4 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-RBP4 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human RBP4
Immunogen
Recombinant Human RBP4 protein (Catalog#10354-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human RBP4 / PRBP (rh RBP4; Catalog#10354-H08H; Met 1-Leu 201; NP_006735.2). RBP4 specific IgG was purified by human RBP4 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-RBP4 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:1000-1:2000
IHC-P 1:250-1:1000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-RBP4 Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of human RBP4 in human pancreas with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).
Immunochemical staining of human RBP4 in human liver with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.
Immunochemical staining of human RBP4 in human kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections). Positive staining was localized to proximal tubule.

RBP4-His was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-RBP4-His rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-RBP4-His rabbit polyclonal antibody,at 1:50 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 21 kDa

Observed band size: 21 kDa

Anti-RBP4 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:23 kDa

Observed band size:21 kDa

Anti-RBP4 Antibody: Alternative Names

Anti-RDCCAS Antibody

RBP4 Background Information

Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).
Full Name
retinol binding protein 4, plasma
References
  • Yang Q, et al. (2005) Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature. 436(7049): 356-62.
  • Choi SH, et al. (2008) High plasma retinol binding protein-4 and low plasma adiponectin concentrations are associated with severity of glucose intolerance in women with previous gestational diabetes mellitus. J Clin Endocrinol Metab. 93(8): 3142-8.
  • Tepper BJ, et al. (2010) Serum retinol-binding protein 4 (RBP4) and retinol in a cohort of borderline obese women with and without gestational diabetes. Clin Biochem. 43(3): 320-3.

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