PD-L2 Matched ELISA Antibody Pair Set,Human

1/1
Price:
Size:
Number:

PD-L2 Matched ELISA Antibody Pair Set,Human General Information

Product name
PD-L2 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human PD-L2
Assay range
25-1600 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of rabbit anti-Human PD-L2/B7-DC/CD273 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10292-M324)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human PD-L2/B7-DC/CD273 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 70 ng of recombinant Human PD-L2/B7-DC/CD273. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1600 pg/mL is recommended.
Product overview
This PD-L2 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human PD-L2 . It contains Human PD-L2 capture antibody, Human PD-L2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human PD-L2 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

PD-L2 Matched ELISA Antibody Pair Set,Human Images

PD-L2 Matched ELISA Antibody Pair Set,Human: Synonyms

B7-DC Matched ELISA Antibody Pair Set, Human; B7DC Matched ELISA Antibody Pair Set, Human; bA574F11.2 Matched ELISA Antibody Pair Set, Human; Btdc Matched ELISA Antibody Pair Set, Human; CD273 Matched ELISA Antibody Pair Set, Human; PD-L2 Matched ELISA Antibody Pair Set, Human; PDCD1L2 Matched ELISA Antibody Pair Set, Human; PDL2 Matched ELISA Antibody Pair Set, Human

PD-L2 Background Information

Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.
Full Name
programmed cell death 1 ligand 2
Research Areas
References
  • Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8.
  • Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30.
  • Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.