CD86 Matched ELISA Antibody Pair Set,Rat

For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.
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CD86 Matched ELISA Antibody Pair Set,Rat General Information

Product name
CD86 Matched ELISA Antibody Pair Set,Rat
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Rat CD86
Assay range
4.69-300 pg/mL
Materials provided
1. Capture Antibody 0.4 mg/mL of mouse anti-rat CD86 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 80025-MM12)
2. Detection Antibody 0.25 mg/mL mouse anti-rat CD86 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0. 5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 80025-MM10)
3. Standard Each vial contains 19 ng of recombinant rat CD86. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.3 ng/mL is recommended.
Product overview
This CD86 Matched ELISA Antibody Pair Set,Rat is a solid phase sandwich ELISA for quantitative determination of Rat CD86 . It contains Rat CD86 capture antibody, Rat CD86 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Rat CD86 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

CD86 Matched ELISA Antibody Pair Set,Rat Images

CD86 Background Information

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
Full Name
CD86 antigen
Research Areas
References
  • Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.
  • Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.
  • Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.
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