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|Cutting-Edge Cell Culture Technology||>5000 Protein Bulks on Shelf Unmatched Experience||Superior Quality Money Back Guarantee|
|Full services:||From gene cloning/synthesis to purified proteins
|Host Cells:||Choices of Expression Hosts:
Transient Transfection in HEK293 Cells
Transient Transfection in CHO cells
Stable expression in CHO cells
Yeast cell expression system
Insect cell/baculovirus expression system
E. coli. microbial expression system
|Myc Tag Design:||Myc tag (EQKLISEEDL-tag) at N- or C-terminus
|Cleavage Site:||Option to design a cleavage site between the tag and protein
Selection of Cleavage Protease Enzyme: Enterokinase (EK),
HRV-3C (human rhinovirus protease), TEV
|Purification:||Myc-tag (EQKLISEEDL-tag) specific antibody conjugated affinity clumn
Myc-tag Affinity Purification Resin
|Deliverable:||Purified recombinant proteins (quantities to be specified by customer)|
|Quality Specification:||typically >90% purity by SDS page
|Typical Timeline:||8-12 weeks|
• > 300 Staff
• > 80 Bioreactors
• > 30 Purification System
• > 10,000 Mab Production
• > 5000 Protein Bulks
• > 5000 antibody development
• 1-100mg Mab Production in 3-6 weeks
• 1-10 g Mab Production in 6-8 weeks
• Preferred CRO Partner for Multiple Top-10 Pharmas
What is a Myc-tag?
A Myc-tag is a polypeptide protein tag derived from the c-myc gene product that can be fused to either the C- or N-terminus of a protein through recombinant DNA technology. It can facilitate the affinity purification of the tagged protein, as well as the isolation and detection of the tagged protein.
The peptide sequence of the myc-tag is: EQKLISEEDL (or -Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-).
What is the Molecular Wieght of the Myc-tag?
The Myc-tag contains 10 amino acids, and the molecular weight of the Myc-tag (EQKLISEEDL-tag) peptide is 1202 Da.
Why use a Myc-tag for protein expression and production?
Adding a Myc-tag to a protein's C- or N-terminus can facilitate its detection, isolation, and purification by Myc-tag specific antibodies. This is particularly helpful for proteins without a detection antibody or with unknown cellular location. Sino Biological has developed Myc-tag specific antibodies for ELISA, Western Blotting, Immonofluorecence, and Immunoprecipitation detection of the Myc-tagged protein. It can also be conjugated to agarose beads for Myc-tagged protein affinity purification.
How to Purify Myc-tagged Proteins?
Myc-tagged recombinant protein can be affinity
Myc-tag (EQKLISEEDL-tag) has been widely used in recombinant protein expression and production. The Myc-tag allows simple and efficient affinity purification of the tagged protein using Myc-tag specific antibody conjugated to agarose-beads. The Myc-tag (EQKLISEEDL-tag) also can be used for detection in western blot by using a Myc-tag-specific antibody. Myc-tag can be added to either C- or N-terminus of a protein for expression in virtually all expression systems.
Sino Biological has successfully produced many proteins with the Myc-tag and purified with Myc-tag affinity chromatography. We are highly experienced in designing the Myc-tag according to the protein structure and handle any problems arising from protein purification process.
Sino Biological has also developed highly sensitive Myc-tag (EQKLISEEDL-tag) specific antibodies for ...... < Learn More>