Mouse CTLA-4 HEK293 Overexpression Lysate: Product Information
This Mouse CTLA-4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CTLA-4 protein (Cat: 50503-M02H) from the overexpression lysate was verified.
A DNA sequence encoding the extracellular domain of mouse CTLA4 (NP_033973.2) (Met 1-Phe 162) was fused with the Fc region of human IgG1 at the C-terminus.
The secreted recombinant mouse CTLA4/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 368 amino acids and has a predicted molecular mass of 41 kDa. As a result of glycosylation, the apparent molecular mass of rm CTLA4/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).
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