Human DAP10 HEK293 Overexpression Lysate: Product Information
This Human DAP10 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of DAP10 protein (Cat: 14063-H05H) from the overexpression lysate was verified.
A DNA sequence encoding the human HCST (Q9UBK5-1) (Met-Pro48) was fused with Fc region of mouse IgG at the C-terminus.
The recombinant human HCST/mFc is a disulfide-linked homodimer. The reduced monomer comprises 264 amino acids and has a predicted molecular mass of 29.3 kDa. The apparent molecular mass of the protein is approximately 34 in SDS-PAGE under reducing conditions.
Human DAP10 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human DAP10 HEK293 Overexpression Lysate: Synonyms
Human DAP10 Overexpression Lysate; Human KAP10 Overexpression Lysate; Human PIK3AP Overexpression Lysate
DAP10 Background Information
HCST (Hematopoietic Cell Signal Transducer, also known as DAP1) is a Protein Coding gene. HCST belongs to the DAP10 family. It is a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. HCST may be part of the immune recognition receptor complex. This receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs). It may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif.
hematopoietic cell signal transducer
Wu J. et al., 1999, Science. 285 (5428): 730-2.
Karimi M. et al., 2006, J Immunol. 175 (12): 7819-28.
André P. et al., 2004, Eur J Immunol. 34 (4): 961-71.
Add to Cart SuccessfullyAdd to Cart FailedShopping cart is being updated, please waitU.S.A.
Successfully added to cart Please enter catalog numberSubmitted successfullyNetwork ErrorPlease enter your company namePlease enter your namePlease enter your emailPlease enter a valid email addressPlease enter some messageNot found.