Human CD200R HEK293 Overexpression Lysate: Product Information
This Human CD200R overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD200R protein (Cat: 11218-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human CD200R1 (AAI43394.1) extracellular domain (Met 1-Leu 266) was fused with a polyhistidine tag at the C-terminus.
The secreted recombinant human CD200R1 comprises 251 amino acids after removal of the signal peptide and has a predicted molecular mass of 28.2 kDa. As a result of glycosylation, rh CD200R1 migrates as an approximately 50-60 kDa band in SDS-PAGE under reducing conditions.
Human CD200R HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human CD200R HEK293 Overexpression Lysate: Synonyms
Human CD200R Overexpression Lysate; Human HCRTR2 Overexpression Lysate; Human MOX2R Overexpression Lysate; Human OX2R Overexpression Lysate
CD200R Background Information
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cell surface glycoprotein CD200 receptor 1 (CD200R1) is an isoform of CD200 receptors that is expressed on cells of the myeloid lineage. CD200R1 is a receptor for the OX-2 membrane glycoprotein. The receptor-substrate interaction may serve as a myeloid downregulatory signal.
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