Anti-TNF-alpha Magnetic Beads Immunoprecipitation (IP) Kit

Temporarily not available outside of China.
Price:
Size:
Number:

Anti-TNF-alpha Magnetic Beads-IP Kit Product Components

Components Storage
Anti-TNF-alpha Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-TNF-alpha magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-TNF-alpha Magnetic Beads-IP Kit Product Description

The Anti-TNF-alpha magnetic Beads, conjugated with Anti-TNF-alpha antibody, are used for immuneprecipitation (IP) of TNF-alpha proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing TNF-alpha proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound TNF-alpha proteins are dissociated from the magnetic beads using an elution buffer.

Anti-TNF-alpha Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-TNF-alpha Antibody(10602-RP02)
Immunogen
Recombinant Human TNF-alpha / TNFa / TNFSF2 protein (Catalog#10602-HNAE)
Species Reactivity
Human TNF-alpha / TNFa / TNFSF2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human TNF-alpha / TNFa / TNFSF2 (rh TNF-alpha / TNFa / TNFSF2; Catalog#10602-HNAE; NP_000585.2; Val77-Leu233). TNF-alpha / TNFa / TNFSF2 specific IgG was purified by Human TNF-alpha / TNFa / TNFSF2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-TNF-alpha Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-DIFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNF-alphaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNFAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNFSF2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Full Name
tumor necrosis factor
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.