Anti-S6K1/RPS6KB1 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-S6K1/RPS6KB1 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-S6K1/RPS6KB1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-S6K1/RPS6KB1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-S6K1/RPS6KB1 Magnetic Beads-IP Kit Product Description

The Anti-S6K1/RPS6KB1 magnetic Beads, conjugated with Anti-S6K1/RPS6KB1 antibody, are used for immuneprecipitation (IP) of S6K1/RPS6KB1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing S6K1/RPS6KB1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound S6K1/RPS6KB1 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-S6K1/RPS6KB1 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-S6K1/RPS6KB1 Antibody, Rabbit Polyclonal(10099-T38)
Immunogen
Recombinant Human PS6K / RPS6KB1 Protein (Catalog#10099-H09B)
Species Reactivity
Human PS6K / RPS6KB1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PS6K / RPS6KB1 (rh PS6K / RPS6KB1; Catalog#10099-H09B; P23443; Met1-Leu525). PS6K / RPS6KB1 specific IgG was purified by Human PS6K / RPS6KB1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-S6K1/RPS6KB1 Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-p70 S6KAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p70(S6K)-alphaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p70-alphaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p70-S6KALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PS6KALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-RPS6KB1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-S6KALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-S6K-beta-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-S6K1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-STK14AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

S6K1/RPS6KB1 Background Information

PS6K, also known as RPS6KB1, is a serine/threonine-protein kinase. It belongs to the RSK (ribosomal s6 kinase) family. Members of this family function in signal transduction. PS6K is an isoform of p70 ribosomal S6 kinase (S6K). S6K can be activated by mitogenic stimuli such as growth factors, insulin and cytokines. It phosphorylates the ribosomal protein S6. PS6K also phosphorylates other proteins such as elF4B, eEF2K and SKAR. It is a crucial effector of mTOR(rapamycin) signaling. PS6K is dissociated from the EIF3 complex and activated upon mitogenic stimulation, phosphorylation by the mammalian target of mTOR complex 1 (mTORC1). Its active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. PS6K also functions in cell proliferation, cell growth and cell cycle progression.
Full Name
ribosomal protein S6 kinase, polypeptide 1
Related Pathways
  • AKT Signaling Pathway
    AKT Signaling Pathway
  • AMPK Signaling Pathway
    AMPK Signaling Pathway
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
References
  • Panasyuk, et al. (2006) Nuclear export of PS6K II is regulated by protein kinase CK2 phosphorylation at Ser-17. J Biol Chem. 281(42):31188-201.
  • Carnevalli L, et al. (2010) PS6K Plays a Critical Role in Early Adipocyte Differentiation. Dev Cell. 18 (5):763-74.
  • Grove JR, et al. (1991) Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini. Mol Cell Biol. 11(11):5541-50.
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