Anti-Clusterin Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-Clusterin Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Clusterin Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Clusterin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Clusterin Magnetic Beads-IP Kit Product Description

The Anti-Clusterin magnetic Beads, conjugated with Anti-Clusterin antibody, are used for immuneprecipitation (IP) of Clusterin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Clusterin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Clusterin proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Clusterin Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-Clusterin Antibody(11297-T04)
Immunogen
Recombinant Human Clusterin/Apolipoprotein J/Apo-J Protein (Catalog#11297-H08H)
Species Reactivity
Human Clusterin/Apolipoprotein J/Apo-J
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Clusterin/Apolipoprotein J/Apo-J (rh Clusterin/Apolipoprotein J/Apo-J; Catalog#11297-H08H; NP_001822.2; Met1-Glu501). Clusterin/Apolipoprotein J/Apo-J specific IgG was purified by Human Clusterin/Apolipoprotein J/Apo-J affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Clusterin Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-AAG4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-APO-JALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-APOJALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CLIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CLU1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CLU2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-KUB1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-NA1/NA2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SGP-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SGP2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SP-40ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TRPM-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TRPM2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Clusterin Background Information

Clusterin, also known as complement-associated protein SP-4, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.
Full Name
clusterin
References
  • Silkensen JR, et al. (1994) The role of clusterin in tissue injury. Biochem Cell Biol. 72(11-12): 483-8.
  • Naik RR, et al. (2002) Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater. 1(3): 169-72.
  • Djeu JY, et al. (2009) Clusterin and chemoresistance. Adv Cancer Res. 105: 77-92.
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