Anti-CDK2 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CDK2 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CDK2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CDK2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CDK2 Magnetic Beads-IP Kit Product Description

The Anti-CDK2 magnetic Beads, conjugated with Anti-CDK2 antibody, are used for immuneprecipitation (IP) of CDK2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CDK2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CDK2 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CDK2 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CDK2 Antibody(10624-RP02)
Immunogen
Recombinant Human CDK2 protein (Catalog#10624-H08B)
Species Reactivity
Human CDK2 / p33
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CDK2 (rh CDK2; Catalog#10624-H08B; NP_001789.2; Met 1-Leu 298). CDK2 specific IgG was purified by human CDK2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CDK2 Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-CDKN2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p33(CDK2)ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CDK2 Background Information

CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. Cdks (cyclin-dependent kinases) are heteromeric serine/threonine kinases that control progression through the cell cycle in concert with their regulatory subunits, the cyclins. Cdks are constitutively expressed and are regulated by several kinases and phosphastases, including Wee1, CDK-activating kinase and Cdc25 phosphatase. Although there are 12 different cdk genes, only 5 have been shown to directly drive the cell cycle (Cdk1, -2, -3, -4, and -6). Following extracellular mitogenic stimuli, cyclin D gene expression is upregulated. Cdk4 forms a complex with cyclin D and phosphorylates Rb protein, leading to liberation of the transcription factor E2F. E2F induces transcription of genes including cyclins A and E, DNA polymerase and thymidine kinase. Cdk4-cyclin E complexes form and initiate G1/S transition. Subsequently, Cdk1-cyclin B complexes form and induce G2/M phase transition. Cdk1-cyclin B activation induces the breakdown of the nuclear envelope and the initiation of mitosis. CDK2 associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. CDK2 is involved in the control of the cell cycle. It also interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
Full Name
cyclin-dependent kinase 2
Related Pathways
  • p53 Pathway
    p53 Pathway
References
  • Bao ZQ, et al. (2011) Briefly bound to activate: transient binding of a second catalytic magnesium activates the structure and dynamics of CDK2 kinase for catalysis. Structure. 19(5):675-90.
  • Neganova I, et al. (2011) An important role for CDK2 in G1 to S checkpoint activation and DNA damage response in human embryonic stem cells. Stem Cells. 29(4):651-9.
  • Li J, et al. (2011) Phosphorylation of MCM3 protein by cyclin E/cyclin-dependent kinase 2 (Cdk2) regulates its function in cell cycle. J Biol Chem. 286(46):39776-85.
  • Buis J, et al. (2012) Mre11 regulates CtIP-dependent double-strand break repair by interaction with CDK2. Nat Struct Mol Biol. 19(2):246-52.
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