Anti-CD146 / MCAM Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD146 / MCAM Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CD146 / MCAM Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD146 / MCAM magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD146 / MCAM Magnetic Beads-IP Kit Product Description

The Anti-CD146 / MCAM magnetic Beads, conjugated with Anti-CD146 / MCAM antibody, are used for immuneprecipitation (IP) of CD146 / MCAM proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD146 / MCAM proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD146 / MCAM proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD146 / MCAM Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CD146 / MCAM Antibody(10115-R005)
Immunogen
Recombinant Human CD146 protein (Catalog#10115-H08H)
Species Reactivity
Human CD146 / MCAM
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 / MCAM extracellular domain (rh CD146; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559) .
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD146 / MCAM Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CD146ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MUC18ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD146 / MCAM Background Information

The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.
Full Name
melanoma cell adhesion molecule
References
  • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
  • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
  • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
  • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
  • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
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