Anti-Galectin 3 / LGALS3 Antibody


Anti-Galectin 3 / LGALS3 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-Galectin 3 / LGALS3 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Galectin 3 / LGALS3
No cross-reactivity in ELISA with
Human Galectin1
Human Galectin8
E.coli cell lysate
Recombinant Human Galectin-3 / LGALS3 protein (Catalog#10289-HNAE)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Galectin-3 / LGALS3 (rh Galectin-3 / LGALS3; Catalog#10289-HNAE; P17931; Met1-Ile250). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #01
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Galectin 3 / LGALS3 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:1000-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Galectin 3 / LGALS3 Antibody (Mouse Monoclonal antibody) Images

Galectin3 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

4 µL anti-Galectin3 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Galectin3 mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 30 kDa

Observed band size: 30 kDa

Anti-Galectin3 mouse monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: SW480 Whole Cell Lysate

Lane C: A431 Whole Cell Lysate

Lane D: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:31 kDa

Anti-Galectin 3 / LGALS3 Antibody: Alternative Names

Anti-CBP35 Antibody; Anti-GAL3 Antibody; Anti-GALBP Antibody; Anti-GALIG Antibody; Anti-L31 Antibody; Anti-LGALS2 Antibody; Anti-MAC2 Antibody

Galectin 3 / LGALS3 Background Information

LGALS3, a member of the lectin family, has an important role in tumor progression through inhibition of apoptosis.The galectin-3 gene (LGALS3) encodes a beta-galactose binding lectin. LGALS3 expression is associated with neoplastic transformation and with differentiation of monocytes to macrophages. Galectin-3 is a beta-galactoside-binding lectin which is involved in modulating inflammation and apoptosis. Elevated expression of galectin-3 has been demonstrated in synovium of rheumatoid arthritis (RA).
Full Name
lectin, galactoside-binding, soluble, 3
  • Dumic J, et al. (2006) Galectin-3: an open-ended story. Biochim Biophys Acta. 1760(4): 616-35.
  • Sharma UC, et al. (2004) Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction. Circulation. 110(19): 3121-8.
  • Yan YP, et al. (2009) Galectin-3 mediates post-ischemic tissue remodeling. Brain Res. 1288: 116-24.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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