MMP-9 ELISA Kit, Rat

Temporarily not available outside of China.Online Datasheet is a general version, for batch specific information please refer to the hard copy in shipping package.
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MMP-9 ELISA Kit, Rat: General Information

Product name
MMP-9 ELISA Kit, Rat
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
10.69 pg/mL
Assay range
31.25-2000 pg/mL
Specificity
Recognizes both recombinant and natural Rat MMP-9
Interference
Preparations of the following factors at 50 ng/mL in a mid-range recombinant Rat MMP9 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 319.00 637.00 1,206.00
SD 7.73 10.67 20.60
CV(%) 2.4% 1.7% 1.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 330.00 653.00 1,281.00
SD 20.36 31.07 56.65
CV(%) 6.2% 4.8% 4.4%
Recovery
The recovery of Rat MMP9 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
81
Range
78 -85%
Linearity
Serum
  Recovery of detected
1:2 90%
1:4 100%
1:8 100%
1:16 92%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This MMP-9 ELISA Kit, Rat is an enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP-9 protein in Serum . It contains recombinant Rat MMP-9, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

MMP-9 ELISA Kit, Rat: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Rat MMP9. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

MMP-9 Background Information

Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions, such as morphogenesis, differentiation, angiogenesis, and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases, and tumor invasion. MMP9, also known as 92-kDa gelatinase B/type IV collagenase, is secreted from neutrophils, macrophages, and some transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. It plays an important role in tissue remodeling in normal and pathological inflammatory processes. MMP-9 is a major secretion product of macrophages and a component of cytoplasmic granules of neutrophils and is particularly important in the pathogenesis of inflammatory, infectious, and neoplastic diseases in many organs including the lung. This enzyme is also secreted by lymphocytes and stromal cells upon stimulation by inflammatory cytokines, or upon delivery of bi-directional activation signals following integrin-mediated cell-cell or cell-extracellular matrix (ECM) contacts. Since the integrity of the tissue architecture is closely dependent on the delicate balance between MMPs and their inhibitors, excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a consequence, regulation of gene transcription and tissue-specific expression of MMP-9 in normal and diseased states are being actively investigated to pave the way for new therapeutic targets. Besides, the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention.
Full Name
matrix metallopeptidase 9
Research Areas
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • St-Pierre Y, et al. (2003) Emerging features in the regulation of MMP-9 gene expression for the development of novel molecular targets and therapeutic strategies. Curr Drug Targets Inflamm Allergy. 2(3): 206-15.
  • St-Pierre Y, et al. (2004) Regulation of MMP-9 gene expression for the development of novel molecular targets against cancer and inflammatory diseases. Expert Opin Ther Targets. 8(5): 473-89.
  • Chakrabarti S, et al. (2005) Matrix metalloproteinase-2 (MMP-2) and MMP-9 in pulmonary pathology. Exp Lung Res. 31(6): 599-621.
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