Cat: KIT10395A
IL-6 ELISA Kit, Human: General Information
Product name
IL-6 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant
Sensitivity
1.24 pg/mL
Assay range
5.47-350 pg/mL
Specificity
Recognizes both recombinant and natural Human IL-6
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL6 control were assayed for interference. No significant interference was observed.
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 66.00 | 126.00 | 230.00 |
| SD | 1.82 | 5.94 | 17.09 |
| CV(%) | 2.7% | 4.7% | 7.4% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 59.00 | 116.00 | 215.00 |
| SD | 7.51 | 10.02 | 18.03 |
| CV(%) | 12.8% | 8.6% | 8.4% |
Recovery
The recovery of Human IL6 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 75 |
| Range |
| 72-78% |
| Sample |
| supernatant (n=3) |
| Average % Recovery |
| 110 |
| Range |
| 97-117% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 96% |
| 1:4 | 104% |
| 1:8 | 98% |
| 1:16 | 98% |
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-6 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-6 protein in Serum,Cell supernatant . It contains recombinant Human IL-6, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
IL-6 ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL6. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL-6 ELISA Kit, Human: Synonyms
BSF2 ELISA Kit, Human; HGF ELISA Kit, Human; HSF ELISA Kit, Human; IFNB2 ELISA Kit, Human; IL-6 ELISA Kit, Human; Interleukin-6 ELISA Kit, Human
IL-6 Background Information
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 13 (gp13) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Full Name
interleukin 6
Research Areas
Related Pathways
-
IL17 signaling pathway
References
- Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
- Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
- Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
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