Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Kit

Temporarily not available outside of China. The substitute is ELISA Pair Set (Cat# SEK11684, SEKA11684).
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Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Kit: General Information

Product name
Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Kit
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Recombinant protein
Sensitivity
47.68 pg/mL
Assay range
62.5-4000 pg/mL
Specificity
Recognizes both recombinant and natural H1N1 Hemagglutinin/HA
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.48 1.00 2.10
SD 0.01 0.03 0.05
CV(%) 2.4% 3.2% 2.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.39 0.75 1.55
SD 0.05 0.09 0.20
CV(%) 12.9% 11.9% 12.9%
Recovery
The recovery of H1N1 HA spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
89
Range
75 -97%
Linearity
Supernatant
  Recovery of detected
1:2 100%
1:4 101%
1:8 98%
1:16 95%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of H1N1 Hemagglutinin/HA protein in Recombinant protein . It contains recombinant H1N1 Hemagglutinin/HA, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Kit: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Hemagglutinin/HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
References
  • White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
  • Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
  • Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
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