ENPP-5 ELISA Kit, Human

Temporarily not available outside of China.Online Datasheet is a general version, for batch specific information please refer to the hard copy in shipping package.
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ENPP-5 ELISA Kit, Human: General Information

Product name
ENPP-5 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
76.03 pg/mL
Assay range
93.75-6000 pg/mL
Specificity
Recognizes both recombinant and natural Human ENPP-5
Interference
Preparations of the ENPP2 and ENPP7 at 60 ng/mL in a mid-range recombinant Human ENPP5 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 727.29 1,628.10 3,126.94
SD 35.94 48.97 84.75
CV(%) 4.9% 3.0% 2.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 833.76 1,608.92 3,131.05
SD 79.91 160.92 129.04
CV(%) 9.6% 10.0% 4.1%
Recovery
The recovery of Human ENPP5 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
89
Range
79 -98%
Linearity
Serum
  Recovery of detected
1:2 100%
1:4 99%
1:8 117%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This ENPP-5 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human ENPP-5 protein in Serum . It contains recombinant Human ENPP-5, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

ENPP-5 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human ENPP5. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

ENPP-5 ELISA Kit, Human: Synonyms

NPP-5 ELISA Kit, Human

ENPP-5 Background Information

ENPP5 is a member of the nucleotide pyrophosphatase/phosphodiesterase family (NPP). It is a family comprised of dimeric enzymes that catalyze the hydrolysis of phosphate diester bonds. There are seven isoforms in NPP family, some of which prefer nucleotide substrates, some of which prefer phospholipid substrates, and others of which prefer substrates that have not yet been determined. NPP also belongs to the alkaline phosphatase (AP) superfamily of enzymes and they are located in the cell membrane and hydrolyze extracellular phosphate diesters to affect a wide variety of biological processes. ENPP5 belongs to a group of nucleotidemetabolizing ectoenzymes, which regulate the availability of extracellular nucleotides. ENPP5 may play a role in neuronal cell communication. However, it lacks nucleotide pyrophosphatase and lysopholipase D activity. It may also be involved in neuronal cell communication. The amino acid sequence of human ENPP5 is 100%, 88%, and 82% identical to that of chimpanzee, dog and mouse/rat. ENPP5 functions in phospholipid metabolism.
Full Name
ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative)
References
  • Strausberg RL, et al. (2002) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.
  • Gerhard DS, et al. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7.
  • Lamesch P, et al. (2007) hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. Genomics. 89(3):307-15.
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