SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit

[FOR RESEARCH USE ONLY.] Online Datasheet is a general version, for batch specific information please refer to the hard copy in shipping package.
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SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit: General Information

Product name
SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sensitivity
46.68 pg/mL
Assay range
93.75-6000 pg/mL
Specificity
Recognizes both recombinant and natural SARS-CoV-2 Coronavirus Nucleocapsid
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Standard Dilution Buffer
7. Sample Lysis Buffer
8. Color Reagent A
9. Color Reagent B
10. Stop Solution
Product overview
This SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of SARS-CoV-2 Coronavirus Nucleocapsid protein in . It contains recombinant SARS-CoV-2 Coronavirus Nucleocapsid, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
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