CD82 ELISA Kit, Human

Temporarily not available outside of China.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.
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CD82 ELISA Kit, Human: General Information

Product name
CD82 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Recombinant protein
Sensitivity
97.61 pg/mL
Assay range
109.38-7000 pg/mL
Specificity
Recognizes both recombinant and natural Human CD82
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 945.34 1,976.84 4,207.15
SD 44.94 43.49 197.11
CV(%) 4.8% 2.2% 4.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,069.57 2,104.13 4,637.37
SD 126.33 154.53 340.99
CV(%) 11.8% 7.3% 7.4%
Recovery
The recovery of Human CD82 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
94
Range
86 -100%
Linearity
Supernatant
  Recovery of detected
1:2 116%
1:4 98%
1:8 97%
1:16 100%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CD82 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD82 protein in Recombinant protein . It contains recombinant Human CD82, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD82 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant Human CD82. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD82 ELISA Kit, Human: Synonyms

4F9 ELISA Kit, Human; C33 ELISA Kit, Human; GR15 ELISA Kit, Human; IA4 ELISA Kit, Human; KAI-1 ELISA Kit, Human; KAI1 ELISA Kit, Human; R2 ELISA Kit, Human; SAR2 ELISA Kit, Human; ST6 ELISA Kit, Human; TSPAN27 ELISA Kit, Human

CD82 Background Information

CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.
Full Name
CD82 molecule
References
  • Malik FA, et al. (2009) KAI-1/CD82, the molecule and clinical implication in cancer and cancer metastasis. Histol Histopathol. 24(4): 519-30.
  • Liu WM, et al. (2006) KAI1/CD82, a tumor metastasis suppressor. Cancer Lett. 240(2): 183-94.
  • Tonoli H, et al. (2006) CD82 metastasis suppressor gene: a potential target for new therapeutics? Trends Mol Med. 11(12): 563-70.
  • Jackson P, et al. (2005) KAI1 tetraspanin and metastasis suppressor. Int J Biochem Cell Biol. 37(3): 530-4.
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