Anti-CD40 Antibody

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Anti-CD40 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-CD40 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD40
Immunogen
Recombinant Human CD40 protein (Catalog#10774-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CD40 extracellular domain (rh CD40; Catalog#10774-H08H; NP_001241.1; Met 1-Arg 193). CD40 specific IgG was purified by human CD40 affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD40 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD40 Antibody (Rabbit Polyclonal antibody) Images

Anti-CD40 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:31 kDa

Observed band size:37 kDa

Anti-CD40 rabbit polyclonal antibody at 1:500 dilution

Lane A: CD40 konckout Hela Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:31 kDa

Observed band size:37 kDa

Anti-CD40 Antibody: Alternative Names

Anti-Bp50 Antibody; Anti-CDW40 Antibody; Anti-p50 Antibody; Anti-TNFRSF5 Antibody

CD40 Background Information

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Full Name
CD40 molecule, TNF receptor superfamily member 5
References
  • van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.
  • Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.
  • Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.
  • Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.
  • Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.

Standard Antibody Development Service

Rabbit MAb

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Rabbit PAb

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Mouse MAb

Rabbit PAb

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