Anti-PVRL1 / NECTIN1 Antibody (PE)

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Anti-PVRL1 / NECTIN1 Antibody (PE) (Mouse Monoclonal antibody) General Information

Product name
Anti-PVRL1 / NECTIN1 Antibody (PE)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human PVRL1 / NECTIN1
Immunogen
Recombinant Human CD111 / Nectin-1 / PVRL1 protein (Catalog#11611-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD111 / Nectin-1 / PVRL1 (rh CD111 / Nectin-1 / PVRL1; Catalog#11611-H08H; NP_002846.3; Met 1-Thr 334) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Source
Monoclonal Mouse IgG2b Clone #09
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
PE
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-PVRL1 / NECTIN1 Antibody (PE) (Mouse Monoclonal antibody) Images

Flow cytometric analysis of anti-human PVRL1 (CD111) on human erythroleukemia cell line TF-1.

Anti-PVRL1 / NECTIN1 Antibody (PE): Alternative Names

Anti-CD111 Antibody; Anti-CLPED1 Antibody; Anti-ED4 Antibody; Anti-HIgR Antibody; Anti-HV1S Antibody; Anti-HVEC Antibody; Anti-nectin-1 Antibody; Anti-OFC7 Antibody; Anti-PRR Antibody; Anti-PRR1 Antibody; Anti-PVRR Antibody; Anti-PVRR1 Antibody; Anti-SK-12 Antibody

PVRL1 / NECTIN1 Background Information

Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.
Full Name
poliovirus receptor-related 1 (herpesvirus entry mediator C)
References
  • Yoon M, et al. (2002) Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry. J Virol. 76(14): 7203-8.
  • Mata M, et al. (2001) HveC (nectin-1) is expressed at high levels in sensory neurons, but not in motor neurons, of the rat peripheral nervous system. J Neurovirol. 7(5): 476-80.
  • Haarr L, et al. (2001) Transcription from the gene encoding the herpesvirus entry receptor nectin-1 (HveC) in nervous tissue of adult mouse. Virology. 287(2): 301-9.

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