The V5 tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5) corresponding to amino acids 95-108. The V5 epitope tag represents the amino acid sequence GKPIPNPLLGLDST. The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Antibodies against V5 epitope tag recognize and detect V5-tagged proteins without a protein-specific antibody or probe.
Sino Biological anti-V5 Tag antibodies can reliably detect recombinant V5-tagged proteins, and every lot of our anti-V5 Tag antibodies are fully validated in various applications, including Western Blotting (WB), Immonofluorecence (IF) and Immunoprecipitation (IP).
|Product description||Application||Catalog# (PDF)||Size||Price||Select|
|Anti-V5 Tag Antibody, Mouse Mab||ICC/IF IF||100378-MM04||50 µL|
|Anti-V5 Tag Antibody, Mouse Mab||WB IP||50 µL|
|Anti-V5 Tag Antibody, Rabbit PAb, Antigen Affinity Purified||WB IP||50 µL|
|Anti-V5 Tag Antibody, Mouse Mab||WB||100378-MM03||50 µL|
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| Immunofluorescence staining of V5-Tag in Hela cells, transfected with pSTEP2-V5-mFABP4-his (Figure A), pSTEP2-mFABP4-V5-his (Figure B), pSTEP2-V5-ARG1-his (Figure C) and pSTEP2-his-ARG1-V5 (Figure D) using anti-V5 Tag antibody (Cat: 100378-MM04).
Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-V5 Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
|V5 tag was immunoprecipitated using:
Lane A: 0.5 mg V5-ARG1-His transfected 293 Whole Cell Lysate
Lane B: 0.5 mg V5-mFABP4-His transfected 293 Whole Cell Lysate
2 µL anti-V5 tag mouse monoclonal antibody (Cat: 100378-MM03) and 15 μl of 50 % Protein G agarose.
Primary antibody: Anti-V5 tag mouse monoclonal antibody,at 1:100 dilution
Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution
|Immunoprecipitation - Anti-V5 Tag Antibody|
Compatible Secondary Antibodies:
> Goat Anti-Mouse IgG Secondary Antibody (HRP) (Cat: SSA007)
> Goat Anti-Mouse IgG-Fc Secondary Antibody (Biotin) (Cat: SSA013)
> Goat Anti-Rabbit IgG Secondary Antibody (Biotin) (Cat: SSA011)
> Goat Anti-Rabbit IgG-Fc Secondary Antibody (HRP) (Cat: SSA003)