PRAK/MAPKAPK5 Proteins, cDNA Clones Research Reagents

MAPKAPK5 (MAPK Activated Protein Kinase 5, also known as MK5; MK-5; PRAK; MAPKAP-K5), located on 12q24.12-q24.13, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. The gene produces a 54220 Da protein composed of 473 amino acids. The protein encoded by this gene is a tumor suppressor and a member of the serine/threonine kinase family. Diseases such as Glioblastoma Mesenchymal Subtype and Dientamoebiasis are associated with MAPKAPK5. The related pathways of MAPKAPK5 include Signaling by GPCR and Regulation of TP53 Activity.
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PRAK/MAPKAPK5 General Information

PRAK/MAPKAPK5 Protein (1)

Species

    PRAK/MAPKAPK5 cDNA Clone (1)

    NM_003668.3

    In expression vector

    untagged

    PRAK/MAPKAPK5 Lysate (1)

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      PRAK/MAPKAPK5 Background

      MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by an extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro. Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novel in vitro substrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.

      PRAK/MAPKAPK5 References

      • Ni H, et al. (1998) MAPKAPK5, a novel mitogen-activated protein kinase (MAPK)-activated protein kinase, is a substrate of the extracellular-regulated kinase (ERK) and p38 kinase. Biochem Biophys. Res Commun. 243 (2): 492-6.
      • Sudo T, et al. (2000) p62 functions as a p38 MAP kinase regulator. Biochem Biophys Res Commun. 269 (2): 521-5.
      • Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99 (26): 16899-903.
      • New L, et al. (2004) Regulation of PRAK subcellular location by p38 MAP kinases. Mol Biol Cell. 14 (6): 2603-16.

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