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Comments for pCMV3-N-GFPSpark®: |
• CMV promoter: bases 250-837 • Enhancer: bases 838-1385 • SV40 early promoter: bases 3969-3338 • Hygromycin ORF: bases 3356-4381 • pUC origin: bases 5024-5697 • Kanamycin ORF: bases 5771-6586 |
Vector Name | pCMV3-N-GFPSpark® |
Vector Size | 6728bp |
Vector Type | Mammalian Expression Vector |
Expression Method | Constitutive, Stable / Transient |
Promoter | CMV |
Antibiotic Resistance | Kanamycin |
Selection In Mammalian Cells | Hygromycin |
Protein Tag | GFPSpark®(GTGAGCAAGGGC……GAGCTGTACAAG) |
Sequencing Primer | Forward: T7(TAATACGACTCACTATAGGG); Reverse: BGH(TAGAAGGCACAGTCGAGG) |
GFPSpark® is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSpark® is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
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