pCMV3-C-OFPSpark® Vector

pCMV3-C-OFPSpark® Physical Map

pCMV3-C-OFPSpark® Physical Map Comments for pCMV3-C-OFPSpark®:
• CMV promoter: bases 250-837
• Enhancer: bases 838-1385
• SV40 early promoter: bases 2987-3356
• Hygromycin ORF: bases 3374-4399
• pUC origin: bases 5042-5715
• Kanamycin ORF: bases 5789-6604

Cloning Sites for ORF Inserting

Cloning Sites for ORF Inserting

Expression Vector pCMV3-C-OFPSpark® Information Description

Vector Name pCMV3-C-OFPSpark®
Vector Size 6746bp
Vector Type Mammalian Expression Vector
Expression Method Constitutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin

OFPSpark® Tag Information

OFPSpark® is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark® is more than twice brighter than mOrange2. Fast OFPSpark® maturation makes it clearly detectable in mammalian cells as early as within 8 hrs after transfection.

OFPSpark® can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSpark® expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSpark® performs well in some fusions and protein labeling applications.