The Myc tag (amino acid sequence: EQKLISEEDL) is a popular epitope tag derived from c-myc gene product that can be fused to a protein through recombinant DNA technology. The Myc epitope tag contains 10 amino acids, and has a molecular weight of 1.2 KDa. Anti-Myc Tag antibodies facilitate the detection, isolation, and purification of Myc-tagged target proteins without a protein-specific antibody or probe.
Sino Biological anti-Myc Tag antibodies can reliably detect recombinant Myc-tagged proteins, and every lot of our anti-Myc Tag antibodies are fully validated, including Western Blotting (WB) and Immunoprecipitation (IP) applications.
|Product description||Application||Catalog# (PDF)||Size||Price||Select|
|Anti-Myc Tag Antibody, Mouse MAb||WB||50 µL|
|Anti-Myc Tag Antibody, Mouse MAb||IP||50 µL|
|*Please tick the checkbox, and add the Anti-Myc Tag Antibody of interest to cart.||Add to Cart|
|Sino Biological||Competitor A||Western Blot analysis of Myc-tagged proteins using Anti-Myc Tag monoclonal antibody from different brands.
Sample: Recombiant Protein
Lane A: 5 ng GST-Myc protein
Lane B: 1.0 ng GST-Myc protein
Lane C: 100 ng Myc-GST protein
Lane D: 20 ng Myc-GST protein
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution
|Anti-MYC Tag Antibody (Cat: 100029-MM08)||Anti-MYC Tag Antibody from Competitor A|
|MYC tag was immunoprecipitated using:
Lane A: 0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate
Lane B: 0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate
2 µL anti-MYC tag mouse monoclonal antibody (Cat. 100029-MM07) and 15 μl of 50% Protein G agarose.
Primary antibody: Anti-MYC tag mouse monoclonal antibody, at 1:100 dilution
Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution
|Immunoprecipitation - Anti-Myc Tag Antibody|
Compatible Secondary Antibodies:
> Goat Anti-Mouse IgG Secondary Antibody (HRP) (Cat: SSA007)
> Goat Anti-Mouse IgG-Fc Secondary Antibody (HRP) (Cat: SSA006)
> Goat Anti-Mouse IgG Secondary Antibody (Biotin) (Cat: SSA014)
> Goat Anti-Mouse IgG-Fc Secondary Antibody (Biotin) (Cat: SSA013)