Sino Biolicial offers a variety of highly optimized pLV-vectors-based lentiviral ORF expression clones for many applications. Sino Biolicial lentiviral vectors are the third generation lentivirus vector, and which contained specific elements to improve transgene expression, virus titer, biosafety.
|5'LTR||U5 long terminal repeat is required for viral packaging and transcription.The 5' LTR promoter is replace by a CMV promoter to preventing replication of the viral sequences.|
|Psi pack||Packaging signal is RNA target site for packaging by Nucleocapsid.|
|RRE||Rev response element binds gag and increases titers by promoting the nuclear export of unspliced viral genomic RNA.|
|cPPT/cTS||Central polypurine tract (includes DNA Flap region) increases nuclear translocation and integration of transduced viral genome efficiency.|
|CMV2 promoter||The enhancer CMV promoter promotes a high level of expression of your gene of interest in a wide variety of cell lines.|
|WPRE||A woodchuck hepatitis virus posttranscriptional regulatory element prevents poly A site readthrough|
|3' LTR||Required for viral reverse transcription; self- inactivating 3' LTR with deletion in U3 region prevents formation of replication-competent viral particles after integration into genomic DNA|
• 17 lentiviral vectors for cDNA cloning with various tags
• Fluorescent protein tags for lentiviral vector titer detection or any other visualization
• Promotor of lentivial vector is enhancer CMV promotor
• All the cDNA / ORF from Sino Biological can be inserted into lentivial vectors
Here is the sequence and map of each vector with different tags.