Lentiviral Vectors Optimized
Lentiviral Vector Core Elements
Sino Biolicial offers a variety of highly optimized pLV-vectors-based lentiviral ORF expression clones for many applications. Sino Biolicial lentiviral vectors are the third generation lentivirus vector, and which contained specific elements to improve transgene expression, virus titer, biosafety.
| Element | Function |
| 5'LTR | U5 long terminal repeat is required for viral packaging and transcription.The 5' LTR promoter is replace by a CMV promoter to preventing replication of the viral sequences. |
| Psi pack | Packaging signal is RNA target site for packaging by Nucleocapsid. |
| RRE | Rev response element binds gag and increases titers by promoting the nuclear export of unspliced viral genomic RNA. |
| cPPT/cTS | Central polypurine tract (includes DNA Flap region) increases nuclear translocation and integration of transduced viral genome efficiency. |
| CMV2 promoter | The enhancer CMV promoter promotes a high level of expression of your gene of interest in a wide variety of cell lines. |
| WPRE | A woodchuck hepatitis virus posttranscriptional regulatory element prevents poly A site readthrough |
| 3' LTR | Required for viral reverse transcription; self- inactivating 3' LTR with deletion in U3 region prevents formation of replication-competent viral particles after integration into genomic DNA |
Lentiviral Vectors for cDNA/ORF cloning
• 17 lentiviral vectors for cDNA cloning with various tags
• Fluorescent protein tags for lentiviral vector titer detection or any other visualization
• Promotor of lentivial vector is enhancer CMV promotor
• All the cDNA / ORF from Sino Biological can be inserted into lentivial vectors
Lentiviral Vectors information
Here is the sequence and map of each vector with different tags.
