Lentiviral ORF Clone / Plasmid
Efficiently Transfected-Lentiviral ORF Clone
Lentivirus, a type of retrovirus, has become the most popular gene transduction tool. Lentiviral vectors can effectively infect neuronal cells, hepatocytes, cardiomyocytes, tumor cells, endothelial cells, stem cells, and other types of cells. For some difficult-to-transfect cells, such as primary cells and non-differentiated cells, the use of lentiviral vectors can greatly increase the transduction efficiency of the target gene. Lentivirus also has obvious advantages in transient transfection and stable strain screening.
In order to meet different experimental needs, Sinobiological provides various forms of lentiviral ORF clone / plasmid with high-efficiency transfection and better biosafety for customers.
Features of lentiviral ORF clone / plasmid
|
|
| Transfected dividing or non-dividing cells, transient or stable expression | |
|
|
| Full length sequence confirmed, quality assurance | |
|
|
| • Untagged • C terminal：Myc, HA, FLAG, His, GFPSpark® and OFPSpark® • N terminal：Myc, HA, FLAG, His, GFPSpark® and OFPSpark® |
|
| The third generation lentivirus vector, which contains specific elements to improve transgene expression, virus titer, biosafety |
|
| Nearly 40,000 molecules covering human, mice, rats and other species to meet specific needs |
|
| Transfer plasmid can be freely combined with other companies' package kit, all can obtain high titer lentivirus particles |
Advantages of lentiviral ORF clone / plasmid
Lentiviral Titer Test
pLV-GFPSpark® plasmid (Cat. No.: LVCV-01) was transfected into 293LT cells and viral fluid was collected 48 hours later. The lentivirus titer was up to 1.27x10^8 TU/ml measured by flow cytometry.
Lentiviral Particles Infect Different Cells
- 293FT(MOI=1)
- MCF7(MOI=5)
- HepG2(MOI=20)
- HT1080 (MOI=6)
- MC38 (MOI=150)
GFPSpark® lentiviral particles infected different cells at different multiplicity of infection (MOI), 72 hours later, observed under fluorescent microscope.
Wide Compatibility
- pLV-GFPSpark® (SB) +
Package Kit (A company)
- pLV-GFPSpark® (SB)+
Package Kit (B company)
The transfer plasmid can be freely combined with other companies' package kit, all can obtain high titer lentivirus particles. A：pLV-GFPSpark® (SB) + Package Kit (A company)；B：pLV-GFPSpark® (SB)+ Package Kit (B company)
Types of lentiviral vectors
Partial list of lentiviral ORF clone / plasmid
More Lentiviral ORF clone / plasmid reagents
| Molecule | Catalog | Tag | Species |
| PD-L1/B7-H1/CD274 | HG10084-ACGLN | C-GFPSpark® | Human |
| PD-L2/B7-DC/CD273 | HG10292-ACGLN | C-GFPSpark® | Human |
| CD80/B7-1 | HG10698-ACGLN | C-GFPSpark® | Human |
| CD86/B7-2 | HG10699-ACGLN | C-GFPSpark® | Human |
| CD28/TP44 | HG11524-ACGLN | C-GFPSpark® | Human |
| CTLA-4/CD152 | HG11159-ACGLN | C-GFPSpark® | Human |
| VEGF/VEGFA | HG11066-ACGLN | C-GFPSpark® | Human |
| HER2/ERBB2 | HG10004-ACGLN | C-GFPSpark® | Human |
| ERBB3/HER3 | HG10201-ACGLN | C-GFPSpark® | Human |
| VEGFR2/KDR/Flk-1/CD309 | HG10012-ACGLN | C-GFPSpark® | Human |
| TNF-alpha/TNFA/TNFSF2 | HG10602-ACGLN | C-GFPSpark® | Human |
| TP53/p53 | HG10182-ACGLN | C-GFPSpark® | Human |
| ANG1/ANGPT1 | HG13667-ACGLN | C-GFPSpark® | Human |
