Use the control vectors to co-transfect along with lentivirus packaging systems to make a recombinant control lentivirus.
|Description||Catalog Number||Vector Sequence|
|GFP Lentivirus Control Plasmids||LVCV-01||Download|
|OFP Lentivirus Control Plasmids||LVCV-02||Download|
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1. The GFP lentivirus control plamid and packaging vectors co-transfect into 293T cell.
2. Harvest lentiviral supernatant 36-72 hours after transfection.
3. Infected the 293FT cells with lentiviral supernatant to measurement of lentivirus titer.
4. Harvest the cells and perform qRT-PCR and flow cytometry to test lentivial virus titer.
|293FT (MOI=1)||MCF7 (MOI=5)||HepG2 (MOI=20)||HT1080 (MOI=6)||MC38 (MOI=150)|
|GFPSpark® lentivirus particles were transduced in different cells at different MOI. Fluorescent images were taken 72 hrs post transduction.|