Immunoprecipitation (IP) Buffers

Sino Biological buffer for immunoprecipitation KIT includs cell lysis buffer, acidity elution buffer,alklin elution buffer, neutralization buffer and polypeptide elution buffer. The formula as following:

IP Buffer

To PBS add, 
10mM EDTA
1%Triton-X 100
1mM PMSF
(To make 50mls, add 1ml of .5mM stock EDTA, 0.5ml of 10% stock of Triton-X, and .5ml of 100mM stock of PMSF) Thaw PMSF before using)

1X-TBST

20mM Tris
150mM NaCl
1% Tween 20, P.H 7.4

Blocking Buffer

5% BSA in TBST
50mls TBST:2.5g BSA