Immunohistochemistry, using the basic principles of immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides and proteins) in tissues, its location, qualitative and quantitative research, called immunohistochemistry (immunohistochemistry) or immunocytochemistry techniques (immunocytochemistry).It combined the specific of immune response with visibility of histochemistry by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, detection of various antigens in a cell, subcellular level, such as proteins, polypeptides, enzymes, hormones, pathogens, and receptors. Immunohistochemical techniques have developed rapidly in recent years. It was limited to immunofluorescence techniques in 1950s, but gradually developed after the 1950s to establish a highly sensitive and more practical immunization enzyme technology.
The liver is a large, meaty organ that sits on the right side of the belly. the liver is reddish-brown in color and feels rubbery to the touch. Normally you can't feel the liver, because it's protected by the rib cage. The liver is considered a gland, because it produces bile, a substance needed to digest fats. Bile's salts break up fat into smaller pieces so it can be absorbed more easily in the small intestine. There are many kinds of liver diseases. Common liver diseases include hepatitis infection, fatty liver disease, and cancer, as well as damage from alcohol, the pain reliever acetaminophen, and some cancer drugs. Liver diseases may be diagnosed by liver function tests–blood tests that can identify various markers. For example, acute-phase reactants are produced by the liver in response to injury or inflammation. A variety of target molecules expressed on liver tissue is used for the clinical diagnosis of liver disease, liver tissue is commonly used in immunohistochemical detection. IHC is an important means to be widely used to detect liver disease.
Different species of liver slices as follows: