IHC Antibody Features: Examinations by Multiple Tissues

Currently most of the antibodies on the market only have one staining picture, usually staining results of an antibody in different tissues are different, because the same target protein may express in different tissues. So can the antibody you buy detect the target protein in tissues you need? This directly affects your experiment results, because the test data provided by the supplier may not as same as what samples you want test, so the more test data provided by the supplier, the more information you can refer to and the greater likelihood of successful in your experiments.

The following table shows the three antibodies against the same target which supply by two vendors were detected in different cancer tissues. The results show that the binding of three antibodies in various tissue samples are different: the same tissue deteted by the different antibodies but the test results is different, some strong positive, some weak positive, and some negative; The results that different tissue detected by a antibody are very different also.

  Vendor 1 Vendor 1 Vendor 2
  Antibody A Antibody B Antibody C
Colorectal cancer - +++ -
Breast cancer - + -
Prostate cancer - - -
Ovarian cancer - + -
Cervical cancer - ++ -
Endometrial cancer - ++ -
Carcinoid - + -
Head and neck cancer - ++ -
Thyoid cancer - ++ -
Glioma - + -
Lymphoma - + -
Lung cancer + ++ -
Melanoma - ++ -
Skin cancer - + -
Testis cancer - ++ -
Urothelial cancer - + -
Renal cancer - + -
Stomach cancer - + -
Pancreatic cancer - ++ -
Liver cancer ++ + -
Notes: levels of antibody staining
+++ : High      ++ : Medium      + : Weak       - : Negative

Rigorous examinations by multiple tissues and samples

ALDH3A1: 12523-T60

Immunochemical staining of human ALDH3A1 in human esophagus with rabbit polyclonal antibody (0.05 µg/mL, formalin-fixed paraffin embedded sections)
Immunochemical staining of human ALDH3A1 in human lung with rabbit polyclonal antibody (0.05 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of human ALDH3A1 in human stomach with rabbit polyclonal antibody (0.05 µg/mL, formalin-fixed paraffin embedded sections).

Cynop53: 90001-R002

Immunochemical staining of cynomolgus p53 in human gastric cancer with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of cynomolgus p53 in human lung cancer with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of cynomolgus p53 in human gastric cancer with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).

TNFRSF10D: 10413-T60

Immunochemical staining of human TNFRSF10D in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of human TNFRSF10D in human liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of human TNFRSF10D in human placenta with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of human TNFRSF10D in human testis with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).