Immunohistochemistry (IHC) Antibody-Cytomembrane Staining

Immunohistochemistry, using the basic principles of immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides and proteins) in tissues, its location, qualitative and quantitative research, called immunohistochemistry (immunohistochemistry) or immunocytochemistry techniques (immunocytochemistry).It combined the specific of immune response with visibility of histochemistry by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, detection of various antigens in a cell, subcellular level, such as proteins, polypeptides, enzymes, hormones, pathogens, and receptors. Immunohistochemical techniques have developed rapidly in recent years. It was limited to immunofluorescence techniques in 1950s, but gradually developed after the 1950s to establish a highly sensitive and more practical immunization enzyme technology.

Subcellular localization refers to the presence site of a protein in the cell, for example, in the nucleus, cytoplasm or cell membrane. If a protein locates in the cell membrane, the membrane will be stained, while other locations will not be stained. Immunohistochemistry is an efficient and accurate method of detecting subcellular localization of a protein.

Cytomembrane staining as follows

ratPVRL2: 80327-T24

Immunochemical staining of rat PVRL2 in rat testis with rabbit polyclonal antibody (3 µg/mL, formalin-fixed paraffin embedded sections).The image showing cytomembrane staining.

CD147: 10186-MM05

Immunochemical staining of human CD147 in human esophagus with mouse monoclonal antibody (10 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell.

E-cadherin: 10204-T58

Immunochemical staining of human E-cad in human liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).The image showing cytomembrane staining.

VSIG2: 11543-RP02

Immunochemical staining of human VSIG2 in human stomach with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).The image showing cytomembrane staining.

CTNNB1: 11279-R021

Immunochemical staining of human CTNNB1 in human skin with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).The image showing cytomembrane staining.