For the density of cells used in immunofluorescence staining requires a proper density, different cells are often required to be not exactly the same. According to previous experimental experience, give you some advice:
1. General, the use of the density of adherent cells to control the growth of 60%-80%;
2. The appropriate cell density can ensure the good cell state, get the right results;
3. The appropriate cell density can ensure that the effect of staining, both to avoid false negative results, but also can get beautiful pictures.