what they most want to know is that whether the picture are good or not good? Which antibody is should be choose?Are different target proteins expressed in different cells in the same location? Different expression of the target protein in the cells of the color should be what appearance? Here some good and bad immunofluorescence results are showed to indicate what is good immunofluorescence result and what is bad or little bad immunofluorescence result.
There are target proteins expressed in different location in some cells are gave as examples. There are some common cells, such as A431, Hela and A549 etc. are showed. There are some results of different expression location in some cells.