Sino Biological Inc. have advanced antibody development platform, including Immunohistochemistry / IHC, Flow Cytometry / FACS, Immunofluorescence / IF, Immunofluorescence / IF/ ICC, Immunoprecipitation / IP, Western Blot / WB and ELISA. Sino Biological Inc. has developed nearly 1700 Immunofluorescence / IF / ICC antibodies, this provides an effective tool for scientists. These antibodies include different research areas, including cancer, stem cells, diagnostic and Biomarkers etc.. The antibodies were extensive validation by multiple cell types, including cancer cells, such as Hela, MCF7 and HepG2 etc., and stem cells, such as HESC (human embryonic stem cell) and MSC (mesenchymal stem cells).
Immunofluorescence / IF / ICC is a laboratory technique that uses antibodies to detect specific peptides or protein antigens in the cell via specific epitopes. Immunofluorescence / IF / ICC allows researchers to evaluate whether or not cells express the target antigen. Moreover, Immunofluorescence / IF / ICC allow researchers to know sub-cellular location of target antigen.
MSCs are mesenchymal stem cells which are multipotent stromal cells. MSCs can differentitate into variety of cell types.MSCs always refer to human mesenchymal stem cell and mouse mesenchymal stem cell. Mesenchymal stem cells (MSCs) is an important research field for clinical therapy and drug discoveries. Related cytokines and growth factors are crucial factors in mesenchymal stem cell medium and are effective tools for mesenchymal stem cell differentiation. Proteins of human-source and mouse-source can meet demand of human mesenchymal stem cell research and mouse mesenchymal stem cell research. MSCs cell line is often used in Immunofluorescence / IF / ICC test.
Immunofluorescence staining of Human CD166 in MSC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD166 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.