Sino Biological Inc. have advanced antibody development platform, including Immunohistochemistry / IHC, Flow Cytometry / FACS, Immunofluorescence / IF, Immunofluorescence / IF/ ICC, Immunoprecipitation / IP, Western Blot / WB and ELISA. Sino Biological Inc. has developed nearly 1700 Immunofluorescence / IF / ICC antibodies, this provides an effective tool for scientists. These antibodies include different research areas, including cancer, stem cells, diagnostic and Biomarkers etc.. The antibodies were extensive validation by multiple cell types, including cancer cells, such as Hela, MCF7 and HepG2 etc., and stem cells, such as HESC (human embryonic stem cell) and MSC (mesenchymal stem cells).
Immunofluorescence / IF / ICC is a laboratory technique that uses antibodies to detect specific peptides or protein antigens in the cell via specific epitopes. Immunofluorescence / IF / ICC allows researchers to evaluate whether or not cells express the target antigen. Moreover, Immunofluorescence / IF / ICC allow researchers to know sub-cellular location of target antigen.
Mouse spleen cells are the cells that are removed from the spleen of mice, and the cells are very good at detecting various related targets in mice. Mouse spleen cells are often used in Immunofluorescence / IF / ICC test.
Immunofluorescence staining of mouse CD274 in mouse splenocytes.Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD274 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.