Immunofluorescence / IF / ICC Antibody-HA tag

Epitope tags and anti-epitope tag antibodies are widely used for purification and detection of recombinant proteins in immunoblotting, immunostaining and immunoprecipitation.Epitope tagging is widely used in the characterization of newly discovered proteins. With the development of biological technology, researchers can by recombinant DNA techniques, to construct containing the target gene and epitope tagged fusion protein, and then through the specific tag antibody on the identification and purification, in order to achieve the research needs.The major categories:Many different epitope tags have been engineered into recombinant proteins. Sino Biological Inc. has antibodies against FLAG®( DYKDDDDK), HA, HIS-tag, c-Myc, GFP, GST, V5, S-tag, T7-tag, E-tag and Glu-Glu tag.

Tag antibody is a special area or group that determines the specificity of the antigen molecule, and is the structure or sequence of the antibody specific binding. With the development of biological technology, researchers can construct the fusion protein containing target gene and epitope tagged by DNA recombination technology, and then identify and purify the specific tag antibody to meet the requirements of the research.

The human influenza hemagglutinin (HA) epitope was derived from the protein on the HA virus. HA tag is the amino acid sequence of a YPYDVPDYA tag and a particularly common tags. HA is tagged onto proteins for study and analysis and often used in fusion protein-related research through the realization of detection of HA-taq for the detection of fusion proteins. Antibody to HA-tag is the key of the most important tools in the detection. The antibody is often used in purification of HA tag-related proteins, in study of the protein interaction and in detection of expression of protein. HA tag monoclonal antibody can be used to detect or identify of HA‐tagged proteins expressed in multiple expression system by a variety of immunoassays.

Catalog: 100028-MM10

Immunofluorescence staining of HA-Tag in 293 cells, transfected with HA-ARG1 (L) or mock-transfected (R). Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with SBI Mouse anti-HA-tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green).

Catalog: 100028-MM10

Immunofluorescence staining of HA-Tag in 293 cells, transfected with HA-ARG1 (Figure A), HA-mFABP4 (Figure B), ARG1-HA (Figure C) and mFABP4-HA (Figure D). Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-HA-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).