Immunofluorescence (IF/ICC) Antibody-Cell Membrane

Sino Biological Inc. have advanced antibody development platform, including Immunohistochemistry / IHC, Flow Cytometry / FACS, Immunofluorescence / IF, Immunofluorescence / IF/ ICC, Immunoprecipitation / IP, Western Blot / WB and ELISA. Sino Biological Inc.'s Immunofluorescence / IF / ICC antibody development platform have two main types of immunofluorescence imaging. Sino Biological Inc. has developed nearly 400 Immunofluorescence / IF / ICC antibodies, this provides an effective tool for scientists. These antibodies include different research areas, including cancer, stem cells, diagnostic and Biomarkers etc.. The antibodies were extensive validation by multiple cell types, including cancer cells, such as Hela, MCF7 and HepG2 etc., and stem cells, such as HESC (human embryonic stem cell) and MSC (mesenchymal stem cells)

Different proteins were expressed in different cells. The expression of protein in the cell mainly includes the cell membrane, cytoplasm and nucleus. But some proteins are also expressed on the cell organelles, such as the Golgi body, lysosome, and so on.

We have prepared many kinds of target proteins that can be identified in the cell membrane, which are involved in different kinds of cells. The results show that our antibodies can be used for the quality control of the target proteins on the cell membrane.

Catalog: 10004-R002

Immunofluorescence staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody ( 10 µg/ml ) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.

Catalog: 50711-R001

Confocal immunofluorescence analysis of mouse CD6 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD6 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).

Catalog: 11039-R014

Confocal immunofluorescence analysis of human CDH2 in HeLa cells with rabbit monoclonal antibody (5 µg/mL). The image showing membrane staining of HeLa cells.

Catalog: 10001-MM08

Immunofluorescence staining of Human EGFR in A431 cells Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human EGFR monoclonal antibody (10 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.