Immunofluorescence (IF/ICC) Antibody-CD Molecule

Background

Immunofluorescence is a powerful technique that utilizes fluorescent-labeled antibodies to detect specific target antigens. Immunofluorescence uses a single, primary antibody, chemically linked to a fluorophore. The primary antibody recognizes the target antigen and binds to a specific epitope. The attached fluorophore can be detected via fluorescent microscopyThe technique is very sensitive and versatile and finds numerous applications in the fields of immunology, cell morphology, genetics, diagnostics and histopathology.
The cluster of differentiation (or cluster of designation), often abbreviated as CD, is a protocol used for the identification and investigation of cell surface molecules present on white blood cells. The cluster of differentiation nomenclature was proposed for the classification of the many monoclonal antibodies generated by different laboratories around the world against epitopes on the surface molecules of leukocytes. Since then, the use of cluster of differentiation has expanded to many other cell types.