Sino Biological Inc. have advanced antibody development platform, including Immunohistochemistry / IHC, Flow Cytometry / FACS, Immunofluorescence / IF, Immunofluorescence / IF/ ICC, Immunoprecipitation / IP, Western Blot / WB and ELISA. Sino Biological Inc. has developed nearly 1700 Immunofluorescence / IF / ICC antibodies, this provides an effective tool for scientists. These antibodies include different research areas, including cancer, stem cells, diagnostic and Biomarkers etc.. The antibodies were extensive validation by multiple cell types, including cancer cells, such as Hela, MCF7 and HepG2 etc., and stem cells, such as HESC (human embryonic stem cell) and MSC (mesenchymal stem cells).
Immunofluorescence / IF / ICC is a laboratory technique that uses antibodies to detect specific peptides or protein antigens in the cell via specific epitopes. Immunofluorescence / IF / ICC allows researchers to evaluate whether or not cells express the target antigen. Moreover, Immunofluorescence / IF / ICC allow researchers to know sub-cellular location of target antigen.
A431 is human epithelial carcinoma cell line which belongs to cancer cells. A431 cells were established from an epidermoid carcinoma in the skin/epidermis of an 85- year-old female patient. A431 cells have been used for a variety of studies in cell biology and in biomedical research. A431 cell line is often used in Immunofluorescence / IF / ICC test.
Immunofluorescence staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SPARCL1 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoplasm.