Anti-His Tag Antibody

Anti-His Tag Antibody

The His tag is a small epitope tag consisting of six to ten histidine (His) residues generally fused to recombinant proteins. It's also known as polyhistidine tag, hexa histidine tag, 6xHis tag, and His6 tag. Anti-His Tag antibodies facilitate the detection or purification of His-tagged target proteins without a protein-specific antibody or probe.

Our purified anti-His Tag antibodies are reliable tools for the detection of His-tagged target proteins, and they can recognize 6×His Tag or 10× His Tag at different positions, including N-terminus, C-terminus or internal. Every lot of our His tag antibodies are fully validated by Western Blotting (WB) and ELISA.

Anti-His Tag Antibody Product List

Product description Application Catalog# (PDF) Size Price Select
His Tag Antibody, Mouse Mab WB 105327-MM02T 50 µL $180
105327-MM02T 100 µL $250
105327-MM02T 500 µL $500
105327-MM02T 1ml $750
105327-MM02T 5ml $2250
His Tag Antibody(HRP), Mouse Mab ELISA WB 105327-MM02T-H 50 µL $270
105327-MM02T-H 100 µL $375
105327-MM02T-H 500 µL $750
105327-MM02T-H 1ml $1125
105327-MM02T-H 5ml $3375
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Anti-His Tag Antibody (Cat: 105327-MM02T) Features

Different positions--Recognizes His tags at N-terminal, C-terminal or internal

Western Blot - Anti-His Tag antibody   Detection of His-tagged proteins by Western Blot using Anti-His Tag monoclonal antibody.
A-G: proteins with His tag at N-terminal
H-M: proteins with His tag at C-terminal
N-P: proteins with His tag at internal

High sensitivity--Detects lower expression levels

Sino Biological Competitor A
Anti-His Tag antibody with High sensitivity Anti-His Tag antibody with High sensitivity
A-E: Different loading quantity of IL2Rα-His protein sample
Sino Biological Competitor A
Anti-His Tag antibody with High sensitivity Anti-His Tag antibody with High sensitivity
A-E: Different loading quantity of AZGP1-His protein sample

Multi-expression systems--Ideal for mammalian cells, insect cells and bacteria

mammalian cells
A: Negative control
B: His-Human LYPLA2 Lysate
C: His-Human AKR1C2 Lysate
D: Human LILRA3-His Lysate
bacteria
A: Negative control
B: Human M2E1-His Lysate
C: His-sSPA Lysate
D: His-Human GH1 Lysate
mammalian cells
A: Negative control
B: His-Human LYPLA2 Lysate
C: His-Human AKR1C2 Lysate
D: Human LILRA3-His Lysate

Broad spectrum--Identifies more his-tagged proteins

Sino Biological
Competitor A
Competitor B

A: insect cell lysate containing His-LYPLA2 protein (0.5μg)
B: insect cell lysate containing His-AKR1C2 protein (0.5μg)
C: HEK293 cell lysate containing His-AKR1C2 protein (20ng)
D: HEK293 cell lysate containing His-LILRA3 protein (200ng)

Selected Citations Using Anti-His Tag Antibody

Hagiwara, M., Kokubu, E., Sugiura, S., Komatsu, T., Tada, H., Isoda, R., ... & Nakashima, M. (2014). Vinculin and Rab5 complex is requited for uptake of Staphyrococcus aureus and interleukin-6 expression. PloS one, 9(1), e87373.
应用: Western Blot (WB)

Compatible Secondary Antibodies:
> Goat Anti-Mouse IgG Secondary Antibody (HRP) (Cat: SSA007)
> Goat Anti-Mouse IgG-Fc Secondary Antibody (HRP) (Cat: SSA006)
> Goat Anti-Mouse IgG Secondary Antibody (Biotin) (Cat: SSA014)
> Goat Anti-Mouse IgG-Fc Secondary Antibody (Biotin) (Cat: SSA013)

Immunoprecipitation / IP Kit:
> MagpoinsTM His-Tag Immunoprecipitation KIT (Cat: TBN001)

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